- The Early Response of Albino Rat Skin to 3, 7-dimethylbenzanthracene
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Dae Hyun Hwang, Young Che Kim
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Korean J Cytopathol. 1971;5(1):13-16.
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- It is a well known fact that the skin of rats, with a few exceptions, refractory to polycyclic hydrocarbons.
The authors tried to observe whether or not the response to dimethylbenzanthracene of albino rats which are widely used in various experiments in our country is similar to that of mice as reported by Mider and Morton (1939) and Clamor and Stowell (1942) in early changes.
It was confirmed that the skin reactions consisted of atrophy of the epidermis, elongation and proliferation of hair follicles, increased rate of mitotic division and squamous metaplasia of epithelial cells of the hair follicles, and cystic dilatation of the skin appendages.
- Autoradiographic Studies of Early Incorporation of L-Leucine-U-14C into the Lung of Burned Mice
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Sang Hak Kang, Young Che Kim
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Korean J Cytopathol. 1971;5(1):39-44.
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- Non-specific pulmonary diseases of the lung new constitute one of the most important medical, social and economic problems of modern civilization. For the causes of non-specific pulmonary diseases it is well documented that the single basic or common property is hypersecretion of branchial mucus. Also it seems to be acceptable that the disturbance of mucus secretion plays a role in the pathogenesis of pulmonary diseases.
Hu(1966) in our laboratory revealed that injury (stress) induced marked regressive changes of respiratory mucosa and decreased mucus secretion. Sue (1969) confirmed by autoradiographic method that injury affect profoundly the respiratory tract and bring forth regressive changes in the ciliated epithelium, goblet cells and mixed glands, and reduce mucus secretion due to the interference of uptake of 35S (Na₂35SO₄) into the goblet cells. Kim (1966) demonstrated the amounts of RNA of the lung tissues is decreased by injury.
Therefore the authors performed the autoradiographic observation of early incorporation of L- leucine-U-l4C into the epithelium both of the main bronchus and bronchioles in both of normal and∼burned mice in order to confirm that injury may affect on protein metabolism of the epithelium of those tissues.
The results were as follows:
In the burned mice, the incorporation rate of L-leucine-U-l4C into the epithelial cells of the main bronchus decreased, and that of the bronchioles increased and reached its maximum later than those of normal mice.
- The Early Response of Adrenalectomized Albino Rat Skin to 3, 7-Dimethylbenzanthracene
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Dae Young Lee, Young Che Kim
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Korean J Cytopathol. 1971;5(1):47-51.
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- It is a well known fact that the skin of rats, wit& a few exceptions, refractory to polycyclic hydrocarbons.
Hwang and Kim (1971) in our laboratory reported that the early skin reactions of amino rats to dimethylbenzanthracene.
Reports concerning the chemically induced tumors have been made clear that large number of them by some investigators were shown to be antigenic and it is suggested that the oncogenesis due to chemical carcinogen may be related to immune mechanism.
On the other hand adrenalectomy causes marked proliferation of lymphoid tissues, which are main organs concerning to immune mechanism.
Therefore the authors tried to observe the response of adrenalectomized albino rat skin to 3, 7-dimethylbenzanthracene.
It was confirmed that the skin reactions in the skin of the adrenalectomized rats painted with 3, 7-dimethylbenzanthracene simultaneously, consisted of a marked atrophy of hair follicles, whereas those of the rats pre- or post- painted, a marked proliferation of hair follicles, proliferation and increased rate of mitotic division of epithelial cells of the hair follicles.
- A Case of Acardiac Fetus
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Sang Ho Kim, Hoon Suk Song, Young Che Kim
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Korean J Cytopathol. 1971;5(1):53-65.
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- The term monster ’fetus acardius’ is the one of the monozygotic twin who is greatly malformed, reduced and has atrophied or rudimentary body component but no heart or very imperfect one, mainly due to abnormal anastomosis of umbilical vessel in the common placenta between normal and malformed twin.
Up today, many cases are reported as the four types, acardius ancephalus, acardius acephalus, acardius acormus and acardius amorphus. Here, we have a good opportunity to study one case of fetus acardius at the Department of Pathology, Catholic Medical College.
- A Case of Silicosis
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Won Ill Kim, Young Che Kim, Em Goung Yun, Kyu Sang Cho
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Korean J Cytopathol. 1971;5(1):67-72.
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- This is a first autopsy case in Korea. The patient was a 48 years old male who had been exposed for about 13 years to dust as a miner.
Both lungs are massive fibrous adhesion to the pleura. The cut surface of the lung shows excess of pigmentation throughout, but striking and distinctive feature is the presence of round nodules. These are dense and black or gray in colour. Microscopically fibrosis extends into the parenchyma between such nodules and eventually convert the area or the entire lobe into a massive fibrous scar, with only small intervening areas of emphysematous lung parenchyma. The X-ray finding of the lungs show a cavity, measuring 3×4cm and silicotic dense shadow.
The silica was confirmed by qualitative test chemically.
- Cytocidal Effect of Immune Lymphocytes on Transplantable Target Tumor Cells (Ehrlich Carcinoma and Sarcoma 180) in Vitro
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Soo Young Lee, Young Che Kim
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Korean J Cytopathol. 1970;4(2):91-97.
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- It is one of remarkable contributions in recent advances on studies of tumor immunity(Klein and Klein, 1962; Prehn, 1963; old and Boyse, 1964). It is most desirable, if immunological treatment by which one can place hope on tumor repression in human subject is adoptive immunization by administration of homologous or heterologous immune lymphoid cells because it has been known that tumor immunity was mostly related to cell-mediated immunity(Coggin, J.H., Larson, V.M., and Hilleman, M.R.,1967).
Littman, Kim and Suk (1968) reported that both UV-killed Sarcoma 180 and Ehrlich carcinoma ascites cells are powerfully potent in immunizing the mouse.
Kim (1970) in our laboratory reported that the anti-rabbit UV-killed Sarcoma 180 and Ehrlich carcinoma ascites tumor cell-lymphocytes are so effective that both of the solid tumors of mice disappeared completely 30 days after the inoculation of tumors, especially in the group where lymphocytes were injected 14 days after tumor inoculation and the mechanism is the cytotoxic effect of sensitized lymphocytes on the target tumor cells.
When this method is applied clinically, it is possible to obtain powerful immune lymphocytes on the target tumor cells by immunization against homologous or heterologous animals. Prior to clinical application, it is desirable to establish the appropriate method of in vitro tumor cell preservation and in vitro cytoxic test for detecting the potency of cell mediated immunity to tumor cells.
- Autoradiographic Studies of Early Incorporation of L-Leucine-U-14C into the Liver and the Kidney of Burned Mice
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Hoon Suk Song, Young Che Kim
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Korean J Cytopathol. 1970;4(2):99-105.
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- Since the Cuthbertson’s report that traumatic injuries caused a marked increase of non-protein nitrogen in the urine, it has been generally believed that the traumatic injuries enhanced the catabolism of the body, and this increased catabolism was induced by the secretion of corticoids following trauma. However, it has also been known that traumatic injuries stimulate and increase the anabolism as well as catabolism(Whipple).
Investigations have been carried out by the members of our department on the mechanism of the inversion of albumin globulin ratio of plasma protein and the elevation of plasma fibrinogen after burn. According to Lee and Kim(1969), the inversion of albumin-globulin ratio of plasma protein after burn is due to the increased synthesis of albumin and its accelerated "turn-over rate, and the coincidental decreased synthesis of fibrinogen and its retarded turn-over rate.
On the other hand, it is well known that both albumin and fibrinogen are produced solely in the liver. Lee(1968) in our laboratories reported that the marked decrease of the incorporation rate of DL-lysine-T(G) into the liver protein in the burned rats, and Kim and Kim (1969) reported that in the burned mice the incorporation rate of L-leucine-U-l4C into the liver protein reached its maximum only 5 minutes after the injection and the curve for the changes of the incorporation rate of L-leucine-U-l4C into the liver protein plotted against time showed the same pattern as in the normal mice.
The authors performed the autoradiographic observation on the incorporation rate of L-leucine-U-l4C into the liver cells and the renal tubular cells in both of normal and burned mice, to confirm of Kim’s studies morphologically.
The following results were obtained:
1. In the burned mice, the labeled rate of L-leucine-U-l4C into the liver cell reached its maximum later than that in the normal mice, on the other hand, in the renal tubular cells the results were in opposite.
2. In the burned mice, the maximum number of grain of L-leucine-U-l4C reached earlier than that in the normal mice, on the other hand, in the renal tubular cells the inverse results were showed.
- Autoradiographic Studies of Early Incorporation of L-Leucine-U-l4C into Acinar Cells of the Salivary Glands and the Pancreas of Burned Mice
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Kong Woo Shin, Young Che Kim
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Korean J Cytopathol. 1970;4(2):107-114.
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- Since the Cuthbertson’s report that traumatic injuries cause a marked increase of non-protein nitrogen in urine, it has been generally believed that the traumatic injuries enhance the catabolism of the body, and the increased catabolism is induced by the hypersecretion of corticoids following the trauma. However, it has also been reported by some in vestigators that injuries stimulate and increase the anabolism as well as catabolism in the protein metabism.
Investigations have been carried out by the members of our department on the mechanism of elevation of plasma fibrinogen after confirmation of its occurrence following the traumatic injuries. They made clear that the inversion of the plasma albumin-globulin ratio of plasma protein after injury was the integral consequence of the decreased albumin amount due to the increased turn-over rate overshadowing the increased anabolism and of the increased fibrinogen amount due to decreased catabolism. Hong(1966) gave traumatic injuries to the pancreas and Im(1966) to the salivary gland, organs which contain large amount of nucleic acid among the body organs, and studied the influence of trauma on enzyme activity and the amount of RNA of those organs and made clear that the traumatic injuries gave rise to a marked increase of amylase activity and amount of ribonucleic acid in the pancreatic cells and a decrease in the amount of ribonucleic acid of the salivary gland.
Therefore the authors performed the autoradiographic observation to evaluate the early incorporation rate of L-leucine-U-l4C into the acinar cells of the carotid gland, the sublingual gland and the pancreas of the burned mice.
The results were as follows:
In the burned mice, the incorporation rate of L-leucine-U-l4C into tile acinar cells of the salivary glands and the Pancreas reached its maximum earlier than that in the normal mice.
- Autoradiographic Studies of Early Incorporation of L-Leucine-U-l4C into the Gastrointestinal Tract of Burned Mice
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Wung Jae Park, Young Che Kim
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Korean J Cytopathol. 1970;4(2):123-131.
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- Florey(1960) and more recently Skoryna(1967) emphasized the intimate relationship between mucin production and peptic ulcer. Skoryna had an idea that "gastric let" or "let mechanism" was the sum of mechanism which protected the gastric wall against mechanical and chemical injuries, and he insisted on the involvement of mucin production in this Mechanism.
Selye(1950) reported about gastric ulcer following injuries, but he did not mention mucus secretion in the formation of gastric ulcer in the systemic stress. Kim(1965) revealed that injury induced not only regressive changes in the gastric mucosa but also marked decrease of mucus secretion, and Kim and Kim(1968) applied the autoradiographic technique with radioactive 35S (Na35₂SO₄) to the mucus secretion following systemic injury and demonstrated that injury induced marked regressive changes and also marked decrease of mucin production both in gastric mucosa and rectum.
Therefore the authors performed the autoradiographic observation of early incorporation of L-leucine-U-14C into the gastrointestinal tract in both of normal and burned mice in order to confirm that injury may act on protein metabolism of the gastrointestinal tract.
The results were as follows:
In the burned mice, the incorporation rate of L-leucine-U-14C into the epithelial cells of the mucosa both of the esophagus and the duodenum reached its maximum earlier and that of the stomach, the small intestine and the colon later than those of normal mice. Brunner's glandular cells of the duodeum were enhanced the protein synthesis following injuries.
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